"Lec 5 - Cell Culture Engineering" Frontiers of Biomedical Engineering (BENG 100) Professor Saltzman reviews the concept of gene therapy, and gives some examples of where this is applied. Methods to help deliver DNA into cells using viruses and cationic lipids are discussed, as a way to overcome some challenges in gene therapy. Next, Professor Saltzman gives a brief introduction into bacterial and mammalian cell physiology. He describes the different tissues in the body, the cell development/differentiation process, the anchorage dependence of mammalian cells that allows them to form an organism, and the extracellular matrix. 00:00 - Chapter 1. Applications of Gene Transfer 06:40 - Chapter 2. Gene Therapy 14:45 - Chapter 3. Potentials and Limits of Hijacking Viruses 22:28 - Chapter 4. Bacterial and Human Cell Physiology 33:43 - Chapter 5. Cellular Division 38:42 - Chapter 6. Cell Differentiation Complete course materials are available at the Open Yale Courses website: http://open.yale.edu/courses This course was recorded in Spring 2008.
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