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Northen Blot

This video shows steps in experiment. Experiment is about what bacteria transcript when growing on glucose. We know that they transcript GAL mRNA growing on galactose. Cells are harvested and mRNA is extracted,electrophoresis is done to separate it according to size. Gel is Northen blotted to transfer RNA segments to membrane. Membrane is incubated with GAL DNA probe (radio labeled) and binded to mRNA which codes for GAL. After that x-ray film is made and bands are detected.

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Channels: Experiments Biochemistry Microbiology

Tags: nouthernBlot RNA DNA

Uploaded by: ( Send Message ) on 27-05-2007.

Duration: 1m 35s