Microarray hybridization video

Microarray hybridization. The use of microarrays allows the identification of organisms in an ecological sample by hybridizing DNA extracted from the sample to a set of known DNA specimens arrayed on a solid support, usually a microscope slide (reviewed in Call et al. 2003). The sample DNA, or more usually PCR product derived from it, is labeled with a fluorophore so that the specific sites of hybridization on the microarray can be visualized and the intensity of the hybridization signal quantified. The method is conceptually identical to Southern hybridization of a dot blot, but technological innovations allow thousands of known DNA samples to be arrayed on a single microscope slide and many such slides to be produced in a single batch at low cost. Microarray hybridization has been used to assess microbial diversity and to assay for specific pathogenic bacteria in numerous studies (reviewed in Ye et al. 2001; Call et al. 2003). REad more: http://jbpc.mbl.edu/wheelbase/ToolsTechniques/Microarray_Hybridization.html

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Channels: Biochemistry

Tags: Microarray hybridization

Uploaded by: ( Send Message ) on 24-11-2010.

Duration: 1m 1s