Following PCR, you need to get rid of excess short primers, dNTPs, enzymes, short-failed PCR products and salts. We use a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O to get clean PCR products for downstream applications.
Channels: Experiments Biochemistry Cell Biology
Tags: PCR Purification Abnova antibody protein cell biology
Uploaded by: abnova ( Send Message ) on 22-03-2010.
Duration: 3m 4s