Cell Nuclei Protein Extraction

This protocol involves three steps to prepare nuclear extracts. First, the cells are collected. Then, make cell membrane fragile using a pestle in hypotonic buffer. After collection of the cytoplasmic fraction, the nuclei are lysed and the nuclear proteins are solubilized in the lysis buffer.

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Channels: Experiments Biochemistry Cell Biology

Tags: Cell Nuclei Protein Extraction Abnova antibody protein cell biology

Uploaded by: ( Send Message ) on 01-06-2010. Dnatube suggest users to have interest in drug testing, mesothelioma, insurance, medical lawyers.

Duration: 4m 49s

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