by Abnova - Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.
Channels: Experiments Biochemistry Cell Biology
Tags: Abnova antibody cell biology protein covalently coupling Protein
Uploaded by: abnova ( Send Message ) on 13-05-2010.
Duration: 4m 52s