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His-tagged Protein Purification

( http://www.abnova.com ) - Proteins with histidine tag can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This video provides generic protocol to purify 6xHis-tagged protein by Nickel nitrilotriacetic (Ni-NTA) sepharose. More videos at Abnova ( http://www.abnova.com ).

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Channels: Experiments Biochemistry Cell Biology

Tags: His-tagged Protein Purification Abnova antibody protein cell biology

Uploaded by: ( Send Message ) on 13-10-2010. Dnatube suggest users to have interest in drug testing, mesothelioma, insurance, medical lawyers.

Duration: 4m 28s

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