Invitrogen LanthaScreen® Eu Kinase Binding Assay Capabilities Video. The principle of the LanthaScreen® Eu Kinase Binding Assay is shown in Figure 1. Binding of an Alexa Fluor® conjugate or “tracer” to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer and antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix-and-read assay, with no development steps
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Tags: LanthaScreen
Uploaded by: watchme ( Send Message ) on 24-11-2010.
Duration: 9m 59s