Plasmid DNA Extraction (Megaprep)

A plasmid DNA extraction is a method used to extract and purify plasmid DNA. Kits are available from varying manufacturers, which are named by size of bacterial culture. Megaprep is used when the starting E. coli culture volume is 500 ml~2.5 L of LB broth and the expected DNA yield is 1.5~2.5 mg.

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