Plasmid DNA Extraction (Midiprep)

A plasmid DNA extraction is a method used to extract and purify plasmid DNA. Kits are available from varying manufacturers, which are named by size of bacterial culture. “Midiprep” is used when the starting E. coli culture volume is 15-25 ml of LB broth and the expected DNA yield is 100-350 μg.

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