In this video, PKC activation pathway is animated. Upon activation, protein kinase C enzymes are translocated to the plasma membrane by RACK proteins that are actually a membrane-bound receptors for activated protein kinase C proteins. The protein kinase C enzymes are known for their long-term activation. They remain activated after the original activation signal or the Ca2+-wave is gone. This is presumably achieved by the production of diacylglycerol from phosphatidylinositol by a phospholipase. Fatty acids may also play a role in long-term activation.