Physics 111: Optical Trapping (OTZ) Physics 111 Advanced Laboratory. Professor Jan Liphardt This video accompanies the Optical Trapping Experiment, providing students with an introduction to the theory, apparatus, and procedures. An optical trap or optical tweezers is a device used to apply pico-newton sized forces and make precise measurements on a scale of roughly one micron. It can be created by applying a precisely focused laser through a microscope objective onto a dielectric material such as a cell or a Silica bead. It allows scientists to make very detailed manipulations and measurements on very small objects, and thus is a very important tool in biophysics. Optical traps are used in biological experiments ranging from cell sorting to the unzipping of DNA and also in physical applications such as atom cooling. In this experiment, you will calibrate the trap's position and force detection by trapping Silica beads and using the physics of Brownian motion. You will then use the apparatus to manipulate and study the motion of swimming E. coli cells and the transport of vesicles inside a living onion cell. http://advancedlab.org
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Tags: Physics 111: Optical Trapping (OTZ)
Uploaded by: berkeleyphy111 ( Send Message ) on 19-09-2012.
Duration: 39m 21s
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Lec 1 - Physics 111: Atomic Physics (ATM) Part 1. Balmer Series
Lec 2 - Physics 111: Atomic Physics (ATM) Part 2. Zeeman Effect
Lec 3 - Physics 111: Beta Ray Spectroscopy (BRA)
Lec 4 - Physics 111: Brownian Motion in Cells (BMC)
Lec 5 - Physics 111: Instrumentation Section Lab Equipment (BSC)
Lec 6 - Physics 111: Bubble Chamber (BBC)
Lec 7 - Physics 111: Carbon Dioxide Laser (CO2)
Lec 8 - Physics 111: Compton Scattering (COM)
Lec 9 - Physics 111: Gamma Ray Spectroscopy (GMA)
Lec 10 - Physics 111: Hall Effect In A Plasma (HAL)
Lec 11 - Physics 111: Holography (HOL)
Lec 12 - Physics 111: Introduction to Error Analysis
Lec 13 - Physics 111: Josephson Junction Effect (JOS)
Lec 14 - Physics 111: Radiation and Laboratory Safety
Lec 15 - Physics 111: Laser Safety
Lec 16 - Physics 111: Atomic Physics (ATM) Theory Lecture ONLY
Lec 17 - Physics 111: Energy Levels Lecture Part 1
Lec 18 - Physics 111: Energy Levels Lecture Part 2
Lec 19 - Physics 111 Light Sources and Detectors Lecture
Lec 20 - Physics 111: Optical Instruments Lecture
Lec 21 - Physics 111: Energy Transitions Lecture Series
Lec 22 - Physics 111: Laser Induced Fluorescence and Raman Scattering (LIF)
Lec 23 - Physics 111: Low Light Signal Measurements (LLS)
Lec 24 - Physics 111: Non-Linear Spectroscopy and Magneto-Optics Part 1 (MNO)
Lec 25 - Physics 111: Non-Linear Spectroscopy and Magneto-Optics Part 2 (MNO)
Lec 26 - Physics 111: Atom Trapping (MOT)
Lec 27 - Physics 111: Muon Lifetime (MUO)
Lec 28 - Physics 111: Non-Linear Dynamics and Chaos (NLD)
Lec 29 - Physics 111: Nuclear Magnetic Resonance (NMR) Part-1 Continuous Wave
Lec 30 - Physics 111: Nuclear Magnetic Resonance Part-2 Pulsed NMR
Lec 31 - Physics 111: Optical Pumping (OPT)
Lec 32 - Physics 111: How to do an Oral Report
Lec 34 - Physics 111: Rutherford Scattering (RUT)
Lec 35 - Physics 111: Hall Effect In A Semiconductor