3049 views

Lec 33 - Physics 111: Optical Trapping (OTZ)

Physics 111: Optical Trapping (OTZ) Physics 111 Advanced Laboratory. Professor Jan Liphardt This video accompanies the Optical Trapping Experiment, providing students with an introduction to the theory, apparatus, and procedures. An optical trap or optical tweezers is a device used to apply pico-newton sized forces and make precise measurements on a scale of roughly one micron. It can be created by applying a precisely focused laser through a microscope objective onto a dielectric material such as a cell or a Silica bead. It allows scientists to make very detailed manipulations and measurements on very small objects, and thus is a very important tool in biophysics. Optical traps are used in biological experiments ranging from cell sorting to the unzipping of DNA and also in physical applications such as atom cooling. In this experiment, you will calibrate the trap's position and force detection by trapping Silica beads and using the physics of Brownian motion. You will then use the apparatus to manipulate and study the motion of swimming E. coli cells and the transport of vesicles inside a living onion cell. http://advancedlab.org

Video is embedded from external source so embedding is not available.

Video is embedded from external source so download is not available.

Channels: Physics (General)

Tags: Physics 111: Optical Trapping (OTZ)

Uploaded by: ( Send Message ) on 19-09-2012.

Duration: 39m 21s

No content is added to this lecture.

Go to course:

This video is a part of a lecture series from of berkeley

Lecture list for this course

Lec 1 - Physics 111: Atomic Physics (ATM) Part 1. Balmer Series

Lec 2 - Physics 111: Atomic Physics (ATM) Part 2. Zeeman Effect

Lec 3 - Physics 111: Beta Ray Spectroscopy (BRA)

Lec 4 - Physics 111: Brownian Motion in Cells (BMC)

Lec 5 - Physics 111: Instrumentation Section Lab Equipment (BSC)

Lec 6 - Physics 111: Bubble Chamber (BBC)

Lec 7 - Physics 111: Carbon Dioxide Laser (CO2)

Lec 8 - Physics 111: Compton Scattering (COM)

Lec 9 - Physics 111: Gamma Ray Spectroscopy (GMA)

Lec 10 - Physics 111: Hall Effect In A Plasma (HAL)

Lec 11 - Physics 111: Holography (HOL)

Lec 12 - Physics 111: Introduction to Error Analysis

Lec 13 - Physics 111: Josephson Junction Effect (JOS)

Lec 14 - Physics 111: Radiation and Laboratory Safety

Lec 15 - Physics 111: Laser Safety

Lec 16 - Physics 111: Atomic Physics (ATM) Theory Lecture ONLY

Lec 17 - Physics 111: Energy Levels Lecture Part 1

Lec 18 - Physics 111: Energy Levels Lecture Part 2

Lec 19 - Physics 111 Light Sources and Detectors Lecture

Lec 20 - Physics 111: Optical Instruments Lecture

Lec 21 - Physics 111: Energy Transitions Lecture Series

Lec 22 - Physics 111: Laser Induced Fluorescence and Raman Scattering (LIF)

Lec 23 - Physics 111: Low Light Signal Measurements (LLS)

Lec 24 - Physics 111: Non-Linear Spectroscopy and Magneto-Optics Part 1 (MNO)

Lec 25 - Physics 111: Non-Linear Spectroscopy and Magneto-Optics Part 2 (MNO)

Lec 26 - Physics 111: Atom Trapping (MOT)

Lec 27 - Physics 111: Muon Lifetime (MUO)

Lec 28 - Physics 111: Non-Linear Dynamics and Chaos (NLD)

Lec 29 - Physics 111: Nuclear Magnetic Resonance (NMR) Part-1 Continuous Wave

Lec 30 - Physics 111: Nuclear Magnetic Resonance Part-2 Pulsed NMR

Lec 31 - Physics 111: Optical Pumping (OPT)

Lec 32 - Physics 111: How to do an Oral Report

Lec 34 - Physics 111: Rutherford Scattering (RUT)

Lec 35 - Physics 111: Hall Effect In A Semiconductor

Lec 36 - Physics 111: Soldering Technique

Lec 37 - Physics 111: X-Ray Crystallography