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Antibody Purification (Affinity)

Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix.

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Channels: Experiments Biochemistry Cell Biology

Tags: Antibody Purification Affinity Abnova cell protein biology

Uploaded by: ( Send Message ) on 27-04-2010. Dnatube suggest users to have interest in drug testing, mesothelioma, insurance, medical lawyers.

Duration: 6m 40s

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