PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA, (3) ligation into a plasmid vector, and (4) transformation into bacteria and then bacteria will replicate the plasmid.
Channels: Experiments Biochemistry Cell Biology
Tags: PCR Cloning Abnova antibody protein cell biology
Uploaded by: abnova ( Send Message ) on 31-03-2010.
Duration: 9m 48s