DNA ligation joins two linear DNA fragments together with covalent phosphodiester bonds between 3 hydroxyl ends of one nucleotide with the 5 phosphate end of another. In PCR cloning, insert DNA and vector digested by the same restriction enzyme can be ligated by DNA ligase.
Channels: Experiments Biochemistry Cell Biology
Tags: PCR Cloning III Ligation Abnova antibody protein cell biology
Uploaded by: abnova ( Send Message ) on 29-03-2010.
Duration: 1m 51s