The procedure of PCR cloning includes DNA preparation, digestion, ligation and transformation. This video shows the first part- insert PCR. Compared to other Taq-generated PCR inserts, Pfu DNA polymerase-generated PCR fragments will have fewer errors and is more commonly used for molecular cloning.
Channels: Experiments Biochemistry Cell Biology
Tags: Cloning-Insert PCR Abnova antibody protein cell biology
Uploaded by: abnova ( Send Message ) on 22-03-2010.
Duration: 1m 21s