Contruction of Recombinant DNA

Here is some information about recombinant dna: Recombinant DNA (rDNA) is an artificial DNA sequence resulting from the combining of two other DNA sequences in a plasmid. A recombinant protein is a protein produced by an organism after the relevant DNA is inserted into its genome (that is, by a genetically modified organism). This recombines the DNA of two different organisms. Recombinant DNA technique was discovered by Stanley Norman Cohen and Herbert Boyer in 1973. They published their findings in November 1973 in the paper “Construction of Biologically Functional Bacterial Plasmids in vitro”, which described a technique to isolate and amplify genes, or DNA segments, and insert them into another cell with precision creating a Transgenic Bacteria. Recombinant DNA technology was made possible by the discovery of restriction endonucleases by Werner Arber, Daniel Nathans, and Hamilton Smith, for which they received the 1978 Nobel Prize in Medicine. The term recombinant DNA refers to a new combination of DNA molecules that are not found together naturally. Although processes such as crossing over (genetic recombination) technically produce recombinant DNA, the term is generally reserved for DNA produced by joining molecules derived from different biological sources. Text Reff: http://en.wikipedia.org/wiki/Recombinant_DNA

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Channels: Scientific Animations Genetics

Tags: plasmid recombinant DNA technology cloning bacteria

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