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A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA. The enzyme makes two incisions, one through each of the sugar-phosphate backbones (i.e., each strand) of the double helix without damaging the nitrogenous bases. The chemical bonds that the enzymes cleave can be reformed by other enzymes known as ligases, so that restriction fragments carved from differe...
Added: 1089 days ago From tubeman | global.duration: 46.97 | Views: 12587 | Comments: 0
   

Oligonucleotide synthesis is a relatively simple process. Oligonucleotide synthesis is used to make primers crucial in PCR technology. Making a custom oligonucleotide is particularly useful because this will only bind to the region of DNA that is complementary to the custom oligonucleotide sequence. Custom oligonucleotide synthesis allows other sequences, such as restriction sites, to be added on ...
Added: 1129 days ago From second | global.duration: 73.00 | Views: 5126 | Comments: 0

Biology > Introduction to Biology/nVideo Lectures - Lecture 15/nTopics covered: /nRecombinant DNA 1/nInstructor: /nProf. Eric Lander/nTranscript - Lecture 15/nGood morning. So, I see we have a lot of parents here. How many parents have we got here? Welcome to the parents. How many of the parents have done the reading for today? [LAUGHTER] Good, because we'll call on the parents too, right? We'll...
Added: 518 days ago From mitlectures | global.duration: 3010.60 | Views: 7326 | Comments: 0
  

Biology > Introduction to Biology/nVideo Lectures - Lecture 16/nTopics covered: /nRecombinant DNA 2/nInstructor: /nProf. Eric Lander/nTranscript - Lecture 16/nGood morning. So, we are going to see if my voice holds up through this lecture today. It is a casualty of having been at Foxborough yesterday, and then staying up rather late watching the Red Sox game. On the whole, both seemed to have co...
Added: 518 days ago From mitlectures | global.duration: 3032.60 | Views: 4648 | Comments: 0
    

Biology > Introduction to Biology/nVideo Lectures - Lecture 17/nTopics covered: /nRecombinant DNA 3/nInstructor: /nProf. Eric Lander/n /nTranscript - Lecture 17/nGood morning. Good morning. I don't know about you, but I can't take too many more nights like this. I confess, I haven't gotten a thing done for so many nights in a row now, but what a game! How many of you saw the game? Excellent. Ver...
Added: 518 days ago From mitlectures | global.duration: 3020.80 | Views: 4170 | Comments: 0
     

Biology > Introduction to Biology/nVideo Lectures - Lecture 21/nTopics covered: /nVirology/Tumor Viruses/nInstructor: /nProf. Robert A. Weinberg/nTranscript - Lecture 21/nGood morning class, nice to see you again. I trust you had a relaxing weekend and a Happy Halloween, whatever a Happy Halloween is. You recall that last time we were talking about the process of cell transformation, and recall,...
Added: 518 days ago From mitlectures | global.duration: 3020.60 | Views: 4036 | Comments: 0

Biology > Introduction to Biology/nVideo Lectures - Lecture 22/nTopics covered: /nImmunology 1/nInstructor: /nProf. Robert A. Weinberg/n /nTranscript - Lecture 22/nToday, we're talking about immunology as you may recall. Let's roll. Yes, let's roll./nAnd by the way, admire your little finger, because most of the planet isn't as gifted as you are. Not everyone was guaranteed so many brains as you...
Added: 518 days ago From mitlectures | global.duration: 2838.93 | Views: 7213 | Comments: 2
     

Biology > Introduction to Biology/nVideo Lectures - Lecture 20/nTopics covered: /nCancer/nInstructor: /nProf. Robert A. Weinberg/nTranscript - Lecture 20/nSo, when I first came to town, the Red Sox hadn't won for 42 years. Now it's been 86 years, so the whole town all of a sudden changes its psychology. A time for great celebration, at least for you Red Sox fans. At the, after last lecture I had...
Added: 518 days ago From mitlectures | global.duration: 2957.73 | Views: 5986 | Comments: 0
     

Alu sequence is a short stretch of DNA originally characterized by the action of the Alu restriction endonuclease. Alu sequences of different kinds occur in large numbers in primate genomes. In fact, Alu sequences are the most abundant mobile elements in the human genome. They are derived from the small cytoplasmic 7SL RNA, a component of the signal recognition particle. The event, when a copy of ...
Added: 421 days ago From sikadi | global.duration: 209.00 | Views: 3540 | Comments: 0

Digestion is the process of cutting DNA molecules with restriction endonucleases. These special enzymes recognize specific sequences in the DNA molecule (for example GATATC) wherever that sequence occurs in the DNA. In PCR cloning, insert and vector are cutted by the same restriction endonucleases and then join together in the next step, ligation.
Added: 157 days ago From abnova | global.duration: 108.00 | Views: 878 | Comments: 0

DNA ligation joins two linear DNA fragments together with covalent phosphodiester bonds between 3 hydroxyl ends of one nucleotide with the 5 phosphate end of another. In PCR cloning, insert DNA and vector digested by the same restriction enzyme can be ligated by DNA ligase.
Added: 157 days ago From abnova | global.duration: 111.00 | Views: 807 | Comments: 0

The typical cloning workflow involves many steps, particularly, traditional restriction enzyme cloning. In contrast, Gateway recombination cloning uses a one hour, 99%-efficient, reversible recombination reaction, without using restriction enzymes, ligase, and subcloning step. It is an extremely fast and efficient way to clone your gene.
Added: 143 days ago From abnova | global.duration: 238.00 | Views: 833 | Comments: 0
   
 
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Related Tags: restriction Oligonucleotide Recombinant DNA Lecture Virology Tumor Viruses Immunology 1 MIT Cancer Alu repeats PCR Cloning II Digestion Abnova antibody protein cell biology III Ligation Gateway Recombination